Figure 6: Flow Plots in Paroxysmal Nocturnal Hemoglobinuria
In this plot (left), CD64 and side scatter are used to gate on the monocytes.
Analysis of the gated monocyte population (right) identifies a significant population of dim CD14 and dim FLAER cells consistent with a PNH clone. CD14 is a GPI linked cell surface protein. The PNH clone constitutes 94% of monocytes.
Figure 7: Flow Plots in Paroxysmal Nocturnal Hemoglobinuria
Shown on the left is a flow cytometry plot where the red cells are not lysed. Red cells are gated using side scatter and forward scatter. On the right, the red cells are separated based on glycophorin expression (Y-axis) used for gating and CD59 expression (X-axis).
The red cells are separated into three types (I-III) based on the amount of the GPI anchored CD59 protein expression. Type I cells have no deficiency in CD59, Type II cells have partial deficiency in CD59 while Type III cells have complete deficiency of CD59 and are consistent with the PNH clone. Any differences in the clone size between the white blood cells and red blood cells may be due to hemolysis or blood transfusions.
Often, after episodes of hemolysis, red cell clones are smaller than the white cell clones.
Figure 1: Blood in Paroxysmal Nocturnal Hemoglobinuria
A peripheral blood smear of the patient. On low power (left) red cell agglutination can be appreciated (arrows). High power (right) shows red cells with moderate anisopoikilocytosis and red agglutination.
Figure 2: H&E in Paroxysmal Nocturnal Hemoglobinuria
Shown is a bone marrow biopsy from the patient. At low power (left) erythroid hyperplasia is present with a cluster of erythroid precursors (arrow). On higher power (right), the erythroids are left shifted with megaloblastoid features.
Figure 3: CD34 in Paroxysmal Nocturnal Hemoglobinuria
A bone marrow biopsy with immunohistochemical staining for CD34 highlights scattered CD34 positive blasts (arrow) and blood vessels.
Figure 4: Marrow Aspirates in Paroxysmal Nocturnal Hemoglobinuria
Shown is a bone marrow aspirate demonstrating clusters of megaloblastoid erythroid maturation. Note the erythroid nuclear-cytoplasmic asynchrony.
Figure 5: Flow Plots in Paroxysmal Nocturnal Hemoglobinuria
Flow cytometry was performed to identify the PNH clone in the white blood cells. On the left, the granulocytes, monocytes, and lymphocytes are gated using CD15 and side scatter. Of note, the red cells were lysed in this sample. Analysis of the gated granulocytes reveals a large population of dim FLAER and CD24 (arrow) consistent with a PNH clone. FLAER is a fluorescein labeled molecule which binds to GPI anchors on the cell membrane while CD24 is a GPI linked surface protein. Dim expression of these indicates the loss of GPI anchor consistent with a PNH clone. The PNH clone constitutes 97% of all granulocytes.
